Theses and Dissertations at Montana State University (MSU)
Permanent URI for this collectionhttps://scholarworks.montana.edu/handle/1/733
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Item The influences of diet and water systems on rainbow trout gut microbiome in relation to nutrient utilization(Montana State University - Bozeman, College of Agriculture, 2017) Betiku, Omolola Comfort; Chairperson, Graduate Committee: Carl Yeoman; T. Gibson Gaylord and Wendy M. Sealey were co-authors of the article, 'Evaluation of protein reduction and essential amino acids supplementation of plant- and animal protein-based diets on production of rainbow trout (Oncorhynchus mykiss)' submitted to the journal 'Journal of the World Aquaculture Society' which is contained within this thesis.; Carl J. Yeoman, T. Gibson Gaylord, Suzanne L. Ishaq, Glenn C. Duff, Aurelien Mazurie and Wendy M. Sealey were co-authors of the article, 'Divided nutritive function of the rainbow trout (Oncorhynchus mykiss) mid- and hind- gut microbiomes' submitted to the journal 'Frontiers in microbiology' which is contained within this thesis.; Carl J. Yeoman, T. Gibson Gaylord, Glenn C. Duff, Brian Bothner, Stephanie S. Block and Wendy M. Sealey were co-authors of the article, 'Differences in amino acid catabolism by gut microbes with/without prebiotics inclusion in GDDY-based diet affect feed utilization in rainbow trout' submitted to the journal 'Aquaculture' which is contained within this thesis.; Carl J. Yeoman, T. Gibson Gaylord, Ben Americus, Sarah Olivo, Glenn C. Duff and Wendy M. Sealey were co-authors of the article, 'Water system is a controlling variable modulating bacterial diversity of gastrointestinal tract and performance in rainbow trout' submitted to the journal 'Plos one' which is contained within this thesis.Plant protein ingredients are sustainable sources of protein that could be used to meet the demand of the growing aquaculture industry. However, feeding plant protein diets has some drawbacks in terms of reduced growth and poor feed efficiency. This dissertation evaluated the production cost of alternative protein diets for commercial production of rainbow trout. Also, it identified the microbiota and gene functions associated with alternative diets and how they differ between mid- and hind-gut sections of the rainbow trout intestine. Furthermore, it determined differences in microbial community compositions and functions in the luminal and mucosal GIT of trout when fed alternative diets, with/without prebiotics. Lastly, the significance of diet and water as environmental factors shaping the mucosal and luminal bacterial compositions in trout was investigated. Experiment 1 demonstrated that trout growth and body indices were not affected by feeding plant protein diet (PPD). In experiment 2, shotgun metagenomics revealed predominant bacterial population in trout microbiota. Genes related to carbohydrate metabolism were increased in the hindgut intestine of those fed PPD. Experiment 3 demonstrated that replacing fishmeal with 75% GDDY did not alter growth of trout, but not feed intake and feed conversion ratio (FCR). High inclusion of GDDY in trout diet resulted in enrichment of catabolic genes involving branched chain amino acids in trout midgut region. Experiment 4 showed that rearing in a recirculating water system significantly improved trout performance compared to rearing in a flow-through water system, while feed intake and FCR increased in fish raised in the flow-through system. Water samples were more diverse than GIT samples. Bacterial diversity was greater in mucosal scrapings of the GIT than in the lumen. Water system played a major role influencing the microbial communities in trout luminal and mucosal GIT. The lumen shared similar bacteria with the rearing water. The results of this study demonstrated that plant protein can effectively substitute for fishmeal in trout diets. It further showed that trout GIT microbiota vary between the mid- and hind-GIT. The hind-, but not the mid-GIT microbiome appears to be modulated by diet, while the mid-GIT is affected by water system.Item Wetlands and copper concentrations across a headwater catchment impacted by acid rock drainage(Montana State University - Bozeman, 2000) Novak, Tracy M. Knoop; Chairperson, Graduate Committee: Paul HookItem Grazed stubble height along the greenline as a water quality indicator(Montana State University - Bozeman, College of Agriculture, 2003) Finck, Robert Joseph