Publications by Colleges and Departments (MSU - Bozeman)
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Item The study of microbial biofilms by classical fluorescence microscopy(1998) Huang, Ching-Tsan; Stewart, Philip S.; McFeters, Gordon A.Item Consensus model of biofilm structure(1997) Stoodley, Paul; Boyle, John D.; Dodds, I.; Lappin-Scott, H. M.Item Function of bacterial (hyphomonas spp.) capsular exopolymers in biofouling(1997) Weiner, R. M.; Langille, S. E.; Geesey, Gill G.; Quintero, Ernesto J.Item Measuring antimicrobial effects on biofilm bacteria: From laboratory to field(1999) Zelver, Nick; Hamilton, Martin A.; Pitts, Betsey; Goeres, Darla M.; Walker, Diane K.; Sturman, Paul J.; Heersink, JoannaItem Enhanced bacterial biofilm control using electromagnetic fields in combination with antibiotics(1999) McLeod, Bruce R.; Fortun, Susana M.; Costerton, J. William; Stewart, Philip S.Item Fluorescent probes applied to the physiological characterization of bacterial biofilms(1999) Lisle, John T.; Stewart, Philip S.; McFeters, Gordon A.Item Pseudomonas aeruginosa biofilm sensitivity to biocides: use of hydrogen peroxide as a model antimicrobial agent for examining resistance mechanisms(1999) Hassett, Daniel J.; Elkins, James G.; Ma, J.-F.; McDermott, Timothy R.Item Local mass transfer coefficients in bacterial biofilms using fluorescence recovery after photobleaching (frap)(1996) Bryers, James D.; Drummond, F.Pure culture Pseudomonas putida biofilms were cultivated under controlled conditions to desired overall biofilm thicknesses. Said biofilms were placed within half-cell diffusion chambers to estimate, from transient solute concentrations in each chamber, the effective diffusion coefficient for severed macromolecules of increasing molecular weight and molecular complexity. Results of the half-cell studies were found to be erroneous due to the existence of microscopic water channels or crevasses that perforate the polysaccharidic gel matrix of the biofilm, sometimes completely to the supporting substratum. Thus, half-cell devices measure an average transfer coefficient that overestimates the true, local flux of solutes in the biofilm alginate gel matrix. An alternative analytical technique was refined to determine the local diffusion coefficients on a micro-scale in order to avoid the errors created by the water channels. This technique is based upon the Fluorescence Return After Photobleaching (FRAP) which allows image analysis observation of the transport of fluorescently labeled molecules as they migrate into a micro-scale photobleached zone. The technique allowed us to map the local diffusion coefficients of various solute molecules at different horizontal planes and depths in a biofilm. These maps also indirectly indicate the distribution of water channels in the biofilm. FRAP results illustrate a significant reduction in macromolecule transport coefficients in biofilm polymer gel versus the same value in water, with the reduction being dependent on solute molecule size and shape.Item Comparison of retention and expression of recombinant plasmids between suspended and biofilm-bound bacteria degrading tce(1996) Bryers, James D.; Sharp, Robert R.Any exposure of plasmid recombinant microorganisms to an open system environment, either inadvertently or intentionally, mandates research into those fundamental organism:plasmid processes that influence plasmid retention, transfer, and expression. In the open environment, a majority of the microbial activity occurs associated with an interface, within thin biological layers consisting of the cells and their insoluble extracellular polymer; layers known as biofilms. In addition, current toxic wastewater or wastegas treatment reactors exploit bacteria biofilms for certain system operating advantages. Thus any study regarding the fate of recombinant DNA sequences in either an open environment or closed reactor system must consider processes that impact plasmid retention and expression in a biofilm culture. Using recombinant bacteria within a biofilm reactor to degrade a recalcitrant waste requires finding a suitable host to harbor and express the desired degrading plasmid phenotype. Suitable host characteristics include: ability to produce copious amounts of biofilm, resistance to waste-related injury and toxicity, and ability to retain and express the desired plasmid during long term operation. This paper reports on a laboratory evaluation of factors governing plasmid retention and the expression of TCE degradative capacity in both suspended and biofilm cultures.Item Bench-scale/field-scale interpretations: an overview(1995) Cunningham, Alfred B.