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Item Myxospore detection in soil and angler movement in Southwestern Montana : implications for whirling disease transport(Montana State University - Bozeman, College of Letters & Science, 2007) Gates, Kiza Kristine; Chairperson, Graduate Committee: Christopher S. GuyMovement of anglers among rivers in southwestern Montana presents a potential pathway for the spread of whirling disease and other aquatic nuisance species (ANS) on soil laden angling equipment. The objectives of this study were to 1) determine the effectiveness of a density extraction to isolate myxospores from soil and the effectiveness of polymerase chain reaction (PCR) at detecting M. cerebralis myxospores in soil; 2) quantify movement of resident and non-resident anglers in southwestern Montana and soil quantity carried on angling equipment; and 3) determine myxospore adherence to wading equipment materials. Myxospores were extracted from soil using a soil particle density separation technique. A single blind study was used for PCR experiments with varying quantities of myxospores and soil. Angler movement was assessed with a survey at fishing access sites on six southwestern Montana rivers. Soil samples were taken during the survey from boots and waders with a pressure sprayer to assess quantity of soil carried on angling equipment. Myxospore adherence to wading materials (lightweight, neoprene, rubber, and felt) was tested by exposing myxospores to material and rinsing with a pressure sprayer.