Electrokinetic protein extraction from polyacrylamide gels with in- line microfluidic digestion and integrated mass-spectral analysis

dc.contributor.advisorDratz, Edward
dc.contributor.authorGeovanos, Ares
dc.date.accessioned2013-03-05T20:41:46Z
dc.date.available2013-03-05T20:41:46Z
dc.date.issued2013-03
dc.descriptionAbstract Onlyen_US
dc.description.abstractTwo-dimensional polyacrylamide gel electrophoresis (2-D PAGE) remains a commonly used method in proteomics and provides for the detection of changes in protein isoforms as well as comparison and relative quantification of thousands of intact proteins. In conjunction with differential Zdye labeling, spots on the nanogram scale can be detected, but the more dilute samples are subject to losses and contamination over standard multistep preparation procedures, which may lead to failed identifications by mass spectroscopy. We are developing a system that combines the preparatory steps necessary for mass spectral analysis into a fully automated, microfluidic in-line system. The system identifies dilute proteins by the targeted electroextraction of SDS-complexed proteins, followed by in-line tryptic digestion and direct analysis on an Agilent chip-LC.en_US
dc.identifier.urihttps://scholarworks.montana.edu/handle/1/564
dc.language.isoen_USen_US
dc.titleElectrokinetic protein extraction from polyacrylamide gels with in- line microfluidic digestion and integrated mass-spectral analysisen_US
dc.typePresentationen_US
mus.citation.conferenceMSU Student Research Celebration 2012
mus.relation.collegeCollege of Engineering
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
SRC_12-abstract 92.pdf
Size:
325.08 KB
Format:
Adobe Portable Document Format
Description:
abstract only
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
826 B
Format:
Item-specific license agreed upon to submission
Description:
Copyright (c) 2002-2022, LYRASIS. All rights reserved.