Ahluwalia, N.Mastro, A.M.Ball, R.Miles, MaryRajendra, R.Handte, G.2014-09-032014-09-032001Ahluwalia N, AM Mastro, R Ball, MP Miles, R Rajendra, and G Handte. Cytokine production by stimulated mononuclear cells did not change with aging in apparently healthy, well-nourished women. Mechanisms of Ageing and Development, 122:1269-1279, 2001.0047-6374http://dx.doi.org/10.1016/s0047-6374(01)00266-4https://scholarworks.montana.edu/handle/1/3522Aging is often associated with a dysregulation of the immune system. We examined mitogen-stimulated production of interleukin (IL)-2 and proinflammatory cytokines, IL-1β and IL-6, in apparently healthy and generally well-nourished old versus young women. Subjects were screened for health using the SENIEUR protocol and a panel of laboratory tests for inflammation, as well as for the adequacy of nutritional status using criteria related to undernutrition, and protein, iron, vitamin B12, and folate status. Young (n=26, age: 20–40 years) and old (n=44, age: 62–88 years) cohorts did not differ on the number of circulating monocytes, granulocytes, B (CD19+) cells, and T (CD3+, CD4+, and CD8+) cells. No differences (P>0.10) were seen between the two age groups in IL-2, IL-1β and IL-6 levels in whole blood cultures at 48 h after stimulation with PHA (5 mg/l). Furthermore, no age-related differences were noted in the absolute amounts (pg) of IL-1β and IL-6 after normalizing for circulating monocytes, B cells, or T cells (P>0.10). Similarly, no age-related decline in absolute amount of IL-2 (pg) after normalizing for circulating T cells was noted (P>0.10). Thus, contrary to most previous reports, our results do not support an increase in the production of proinflammatory cytokines IL-1β and IL-6, and a reduced production of IL-2 with aging when health and nutritional status are maintained. These findings support our previous results of no change in monocyte function and few alterations in acquired immune response in a carefully selected group of healthy and well-nourished elderly women.Health sciencesArticle