Okabe, SatoshiNielsen, P. H.Characklis, William G.2017-11-022017-11-021992-09S. Okabe, P.H. Nielsen, and W.G. Characklis, "Factors affecting microbial sulfate reduction by desulfovibrio desulfuricans in continuous culture: limiting nutrients and sulfide concentration," Biotechnology and Bioengineering, 40:725-734 (1992).0006-3592https://scholarworks.montana.edu/handle/1/13933The effects of sulfate and nitrogen concentrations of the rate and stoichiometry of microbial sulfate reduction were investigated for Desulfovibrio desulfuricans grown on lactate and sulfate in a chemostat at pH 7.0. Maximum specific growth rates (μmax), half-saturation coefficients (Ksul), and cell yield (Yc/Lac) of 0.344 ± 0.007 and 0.352 ± 0.003 h −1, 1.8 ± 0.3 and 1.0 ± 0.2 mg/L, and 0.020 ± 0.003 and 0.017 ± 0.003 g cell/g lactate, respectively, were obtained under sulfate-limiting conditions at 35°C and 43°C. Maintenance energy requirements for D. desulfuricans were significant under sulfate-limiting conditions. The extent of extracellular polymeric substance (EPS) produced was related to the carbon: nitrogen ratio in the medium. EPS production rate increased with decreased nitrogen loading rate. Nitrogen starvation also resulted in decreased cell size of D. desulfuricans. The limiting C : N ratio (w/w) for D. desulfuricans was in the range of 45 : 1 to 120 : 1. Effects of sulfide on microbial sulfate reduction, cell size, and biomass production were also ivestigated at pH 7.0. Fifty percent inhibition of lactate utilization occurred at a total sulfide concentration of approximately 500 mg/L. The cell size of D. desulfuricans decreased with increasing total sulfide concentration. Sulfide inhibition of D. desulfuricans was observed to be a reversible process.Article