Hiebert, Colin W.Moscou, Matthew J.Hewitt, TimSteuernagel, BurkhardHernandez-Pinzon, InmaGreen, PhonPujol, VincentZhang, PengRouse, Matthew N.Jin, YueMcIntosh, Robert A.Upadhyaya, NarayanaZhang, JianpingBhavani, SridharVrana, JanKarafiatova, MiroslavaHuang, LiFetch, TomDolezel, JaroslavWulff, Brande B. H.Lagudah, EvansSpielmeyer, Wolfgang2021-01-042021-01-042020-02Hiebert, C.W., Moscou, M.J., Hewitt, T. et al. Stem rust resistance in wheat is suppressed by a subunit of the mediator complex. Nat Commun 11, 1123 (2020). DOI: 10.1038/s41467-020-14937-22041-1723https://scholarworks.montana.edu/handle/1/16092Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.en-US© This manuscript version is made available under the CC-BY 4.0 licensehttps://creativecommons.org/licenses/by/4.0Article