Daughenbaugh, Katie F.Martin, MadisonBrutscher, Laura M.Cavigli, IanGarcia, EmmaLavin, MatthewFlenniken, Michelle L.2019-02-132019-02-132015-06Daughenbaugh, Katie, Madison Martin, Laura Brutscher, Ian Cavigli, Emma Garcia, Matt Lavin, and Michelle Flenniken. “Honey Bee Infecting Lake Sinai Viruses.” Viruses 7, no. 6 (June 23, 2015): 3285–3309. doi:10.3390/v7062772.1999-4915https://scholarworks.montana.edu/handle/1/15256Honey bees are critical pollinators of important agricultural crops. Recently, high annual losses of honey bee colonies have prompted further investigation of honey bee infecting viruses. To better characterize the recently discovered and very prevalent Lake Sinai virus (LSV) group, we sequenced currently circulating LSVs, performed phylogenetic analysis, and obtained images of LSV2. Sequence analysis resulted in extension of the LSV1 and LSV2 genomes, the first detection of LSV4 in the US, and the discovery of LSV6 and LSV7. We detected LSV1 and LSV2 in the Varroa destructor mite, and determined that a large proportion of LSV2 is found in the honey bee gut, suggesting that vector-mediated, food-associated, and/or fecal-oral routes may be important for LSV dissemination. Pathogen-specific quantitative PCR data, obtained from samples collected during a small-scale monitoring project, revealed that LSV2, LSV1, Black queen cell virus (BQCV), and Nosema ceranae were more abundant in weak colonies than strong colonies within this sample cohort. Together, these results enhance our current understanding of LSVs and illustrate the importance of future studies aimed at investigating the role of LSVs and other pathogens on honey bee health at both the individual and colony levels.enCC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.https://creativecommons.org/licenses/by/4.0/legalcodeArticle