Nemudraia, AnnaNemudryi, ArtemWiedenheft, Blake2024-08-212024-08-212024Nemudraia, A., Nemudryi, A., & Wiedenheft, B. (2024). Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells. Science, 384(6697), 808-814.0036-8075https://scholarworks.montana.edu/handle/1/18748Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. We show that site-specific RNA breaks generated with type-III CRISPR complexes are repaired in human cells and that this repair can be used for programmable deletions in human transcripts to restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications.en-USCopyright American Association for the Advancement of Science 2024https://perma.cc/57XC-RB6WCRISPR-RNARNAcellular DNAgenome editingArticle10.1126/science.adk5518