Buyukyoruk, MuratWiedenheft, Blake A.2020-04-202020-04-202019-10Buyukyoruk, Murat, and Blake Wiedenheft. “Type I-F CRISPR–Cas Provides Protection from DNA, but Not RNA Phages.” Cell Discovery 5, no. 1 (October 22, 2019). doi:10.1038/s41421-019-0123-9.2056-5968https://scholarworks.montana.edu/handle/1/15850The type I-F CRISPR–Cas system from Pseudomonas aeruginosa (PA14) provides sequence-specific elimination of invading DNA1,2. Recently, a paper published in Cell Research reported that this system targets mRNA through a non-canonical mechanism3. Here, we implement the proposed design rules for generating CRISPR-RNA (crRNA)-guides that target RNA and test whether the proposed RNA-targeting activity provides immunity against RNA phage infection. Our experiments reveal that the type I-F CRISPR system provides protection from DNA, but not RNA phages.CC BY: This license lets you distribute, remix, tweak, and build upon this work, even commercially, as long as you credit the original creator for this work. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials.https://creativecommons.org/licenses/by/4.0/legalcodeArticle