Espinosa-Ortiz, Erika J.Pechaud, YoanLauchnor, Ellen G.Eldon, Rene R.Gerlach, RobinPeyton, Brent M.van Hullebusch, Eric D.Lens, Piet N. L.2016-11-292016-11-292016-06Espinosa-Ortiz, EJ, Pechaud Y, Lauchnor E, Eldon RR, Gerlach R, Peyton BM, van Hullebusch ED , Lens PNL. “Effect of Selenite on the Morphology and Respiratory Activity of Phanerochaete Chrysosporium Biofilms.” Bioresource Technology 210 (June 2016): 138–145. doi:10.1016/j.biortech.2016.02.074.0960-8524https://scholarworks.montana.edu/handle/1/12261The temporal and spatial effects of selenite (SeO32-) on the physical properties and respiratory activity of Phanerochaete chrysosporium biofilms, grown in flow-cell reactors, were investigated using oxygen microsensors and confocal laser scanning microscopy (CLSM) imaging. Exposure of the biofilm to a SeO32- load of 1.67 mg Se L-1 h-1 (10 mg Se L-1 influent concentration), for 24 h, resulted in a 20% reduction of the O2 flux, followed by a ~10% decrease in the glucose consumption rate. Long-term exposure (4 days) to SeO32- influenced the architecture of the biofilm by creating a more compact and dense hyphal arrangement resulting in a decrease of biofilm thickness compared to fungal biofilms grown without SeO32-. To the best of our knowledge, this is the first time that the effect of SeO32- on the aerobic respiratory activity on fungal biofilms is described.Article