Stewart, Philip S.Griebe, ThomasSrinivasan, RohiniChen, Cheng-SaoYu, Feipeng Philipde Beer, DirkMcFeters, Gordon A.2017-06-262017-06-261994-05Stewart, P.S., T. Griebe, R. Srinivasan, C.-I. Chen, F.P. Yu, D. DeBeer, and G.A. McFeters, "Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms," Appl. Environ. Microbiol., 60(5):1690-1692.0099-2240https://scholarworks.montana.edu/handle/1/13144Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experienced a 93% reduction. Integrated measures of biofilm respiratory activity, including net oxygen and glucose utilization rates, showed only a 10 to 15% reduction. In this biofilm system, measures of microbial respiratory activity and culturability yielded widely differing estimates of biocide efficacy.Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilmsArticle