Camper, Anne K.Hayes, J. T.Sturman, Paul J.Jones, Wallace E.Cunningham, Alfred B.2017-06-262017-06-261993-10Camper AK, Hayes JT, Sturman PJ, Jones WL and Cunningham AB, "Effects of motility and adsorption rate coefficient on transport of bacteria through saturated porous media," Appl Environ Microbiol, 1993 59(10):3455-3462.0099-2240https://scholarworks.montana.edu/handle/1/13145Three strains of Pseudomonasfluorescens with different motility rates and adsorption rate coefficients were injected into porous-medium reactors packed with 1-mm-diameter glass spheres. Cell breakthrough, time to peak concentration, tailing, and cell recovery were measured at three interstitial pore velocities (higher than, lower than, and much lower than the maximal bacterial motility rate). All experiments were done with distilled water to reduce the effects of growth and chemotaxis. Contrary to expectations, motility did not result in either early breakthrough or early time to peak concentration at flow velocities below the motility rate. Bacterial size exclusion effects were shown to affect breakthrough curve shape at the very low flow velocity, but no such effect was seen at the higher flow velocity. The tendency of bacteria to adsorb to porous-medium surfaces, as measured by adsorption rate coefficients, profoundly influenced transport characteristics. Cell recoveries were shown to be correlated with the ratio of advective to adsorptive transport in the reactors. Adsorption rate coefficients were found to be better predictors of microbial transport phenomena than individual characteristics, such as size, motility, or porous-medium hydrodynamics.Article