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dc.contributor.authorPurcell, Alicia M.
dc.contributor.authorMikucki, Jill A.
dc.contributor.authorAchberger, Amanda M.
dc.contributor.authorAlekhina, Irina A.
dc.contributor.authorBarbante, Carlo
dc.contributor.authorChristner, Brent C.
dc.contributor.authorGhosh, Dhritiman
dc.contributor.authorMichaud, Alexander B.
dc.contributor.authorMitchell, Andrew C.
dc.contributor.authorPriscu, John C.
dc.contributor.authorScherer, Reed
dc.contributor.authorSkidmore, Mark L.
dc.contributor.authorVick-Majors, Trista J.
dc.identifier.citationPurcell, Alicia M., Jill A. Mikucki, Amanda M. Achberger, Irina A. Alekhina, Carlo Barbante, Brent C. Christner, Dhritiman Ghosh, et al. “Microbial Sulfur Transformations in Sediments from Subglacial Lake Whillans.” Frontiers in Microbiology 5 (November 19, 2014). doi:10.3389/fmicb.2014.00594.en_US
dc.description.abstractDiverse microbial assemblages inhabit subglacial aquatic environments. While few of these environments have been sampled, data reveal that subglacial organisms gain energy for growth from reduced minerals containing nitrogen, iron, and sulfur. Here we investigate the role of microbially mediated sulfur transformations in sediments from Subglacial Lake Whillans (SLW), Antarctica, by examining key genes involved in dissimilatory sulfur oxidation and reduction. The presence of sulfur transformation genes throughout the top 34 cm of SLW sediments changes with depth. SLW surficial sediments were dominated by genes related to known sulfur-oxidizing chemoautotrophs. Sequences encoding the adenosine-5′-phosphosulfate (APS) reductase gene, involved in both dissimilatory sulfate reduction and sulfur oxidation, were present in all samples and clustered into 16 distinct operational taxonomic units. The majority of APS reductase sequences (74%) clustered with known sulfur oxidizers including those within the “Sideroxydans” and Thiobacillus genera. Reverse-acting dissimilatory sulfite reductase (rDSR) and 16S rRNA gene sequences further support dominance of “Sideroxydans” and Thiobacillus phylotypes in the top 2 cm of SLW sediments. The SLW microbial community has the genetic potential for sulfate reduction which is supported by experimentally measured low rates (1.4 pmol cm-3d-1) of biologically mediated sulfate reduction and the presence of APS reductase and DSR gene sequences related to Desulfobacteraceae and Desulfotomaculum. Our results also infer the presence of sulfur oxidation, which can be a significant energetic pathway for chemosynthetic biosynthesis in SLW sediments. The water in SLW ultimately flows into the Ross Sea where intermediates from subglacial sulfur transformations can influence the flux of solutes to the Southern Ocean.en_US
dc.description.sponsorshipNational Science Foundation Office of Polar Programs (NSF-OPP) grants 0838896; 0839059; 0838941; 0838933, as part of the WISSARD Projecten_US
dc.titleMicrobial sulfur transformations in Subglacial Lake Whillans sedimentsen_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentLand Resources & Environmental Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidVick-Majors, Trista J.|0000-0002-6868-4010en_US
mus.contributor.orcidMitchell, Andrew C.|0000-0001-9749-5326en_US

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