Behavioral consequences following AAV mediated hippocampal EAAC1 knockdown
Coombs, Katie Marie
MetadataShow full item record
The neuronal glutamate transporter EAAC1 (EAAT3) is present in hippocampal neurons to prevent excessive glutamate accumulation. Glutamate receptor-dependent synaptic plasticity is important for learning and memory. The present study investigates behavior associated with blocking the glutamate transporter EAAC1. To manipulate EAAC1 function, rats were intrahippocampally injected with a adeno-associated viral (AAV) vector encoding an EAAC1 antisense mRNA sequence or an AAV empty cassette. Twenty-eight days following surgery, rats were tested in a delayed matching-to-place (DMTP) watermaze task to examine spatial memory, which is hippocampaldependent. Rats treated with EAAC1 antisense exhibited shorter latencies to locate the target platform relative to controls (p < 0.05). These data indicate that microinfusion of AAV encoding EAAC1 antisense significantly altered performance on task involving glutamate transmission and the hippocampus.