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dc.contributor.authorVilla, Federica
dc.contributor.authorPitts, Betsey
dc.contributor.authorLauchnor, Ellen G.
dc.contributor.authorCappitelli, Francesca
dc.contributor.authorStewart, Philip S.
dc.identifier.citationVilla F, Pitts B, Lauchnor E, Cappitelli F, Stewart PS, ʺDevelopment of a laboratory model of a phototroph-heterotroph mixed-species biofilm at the stone/air interface,ʺ Frontiers in Microbiology 2015 6:1251.en_US
dc.description.abstractRecent scientific investigations have shed light on the ecological importance and physiological complexity of subaerial biofilms (SABs) inhabiting lithic surfaces. In the field of sustainable cultural heritage (CH) preservation, mechanistic approaches aimed at investigation of the spatiotemporal patterns of interactions between the biofilm, the stone, and the atmosphere are of outstanding importance. However, these interactions have proven difficult to explore with field experiments due to the inaccessibility of samples, the complexity of the ecosystem under investigation and the temporal resolution of the experiments. To overcome these limitations, we aimed at developing a unifying methodology to reproduce a fast-growing, phototroph-heterotroph mixed species biofilm at the stone/air interface. Our experiments underscore the ability of the dual-species SAB model to capture functional traits characteristic of biofilms inhabiting lithic substrate such as: (i) microcolonies of aggregated bacteria; (ii) network like structure following surface topography; (iii) cooperation between phototrophs and heterotrophs and cross feeding processes; (iv) ability to change the chemical parameters that characterize the microhabitats; (v) survival under desiccation and (vi) biocide tolerance. With its advantages in control, replication, range of different experimental scenarios and matches with the real ecosystem, the developed model system is a powerful tool to advance our mechanistic understanding of the stone-biofilm-atmosphere interplay in different environments.en_US
dc.description.sponsorshipM.J. Murdock Charitable Trust | Seventh Framework Programme (328215)en_US
dc.rightsCC BY 4.0en_US
dc.titleDevelopment of a laboratory model of a phototroph-heterotroph mixed-species biofilm at the stone/air interfaceen_US
mus.citation.journaltitleFrontiers in Microbiologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentBiological Sciences.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMathematical Sciences.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US

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