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dc.contributor.authorCarlson, Ross P.
dc.contributor.authorOshota, Olusegun J.
dc.contributor.authorShipman, Matt R.
dc.contributor.authorCaserta, J. A.
dc.contributor.authorHu, P.
dc.contributor.authorSaunders, C. W.
dc.contributor.authorXu, Jun
dc.contributor.authorJay, Zackary J.
dc.contributor.authorReeder, N.
dc.contributor.authorRichards, Abigail M.
dc.contributor.authorPettigrew, Charles
dc.contributor.authorPeyton, Brent M.
dc.date.accessioned2016-11-29T16:30:01Z
dc.date.available2016-11-29T16:30:01Z
dc.date.issued2016-05
dc.identifier.citationCarlson RP, Oshota O, Shipman M, Caserta JA, Hu P, Saunders CW, Xu J, Jay ZJ, Reeder N, Richards A, Pettigrew C, Peyton BM, "Integrated molecular, physiological and in silico characterization of two Halomonas isolates from industrial brine," Extremophiles 2016 May 20(3):261–74.en_US
dc.identifier.issn1431-0651
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12258
dc.description.abstractTwo haloalkaliphilic bacteria isolated from industrial brine solutions were characterized via molecular, physiological, and in silico metabolic pathway analyses. Genomes from the organisms, designated Halomonas BC1 and BC2, were sequenced; 16S ribosomal subunit-based phylogenetic analysis revealed a high level of similarity to each other and to Halomonas meridiana. Both strains were moderate halophiles with near optimal specific growth rates (=60 % µmax) observed over <0.1–5 % (w/v) NaCl and pH ranging from 7.4 to 10.2. Isolate BC1 was further characterized by measuring uptake or synthesis of compatible solutes under different growth conditions; in complex medium, uptake and accumulation of external glycine betaine was observed while ectoine was synthesized de novo in salts medium. Transcriptome analysis of isolate BC1 grown on glucose or citrate medium measured differences in glycolysis- and gluconeogenesis-based metabolisms, respectively. The annotated BC1 genome was used to build an in silico, genome-scale stoichiometric metabolic model to study catabolic energy strategies and compatible solute synthesis under gradients of oxygen and nutrient availability. The theoretical analysis identified energy metabolism challenges associated with acclimation to high salinity and high pH. The study documents central metabolism data for the industrially and scientifically important haloalkaliphile genus Halomonas.en_US
dc.titleIntegrated molecular, physiological and in silico characterization of two Halomonas isolates from industrial brineen_US
dc.typeArticleen_US
mus.citation.extentfirstpage261en_US
mus.citation.extentlastpage274en_US
mus.citation.issue3en_US
mus.citation.journaltitleExtremophilesen_US
mus.citation.volume20en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s00792-015-0806-6en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage7en_US


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