Show simple item record

dc.contributor.authorZhou, Aifen
dc.contributor.authorHillesland, Kristina L.
dc.contributor.authorHe, Zhili
dc.contributor.authorSchackwitz, Wendy
dc.contributor.authorQichao, Tu
dc.contributor.authorZane, Grant M.
dc.contributor.authorQiao, Ma
dc.contributor.authorQu, Yuanyuan
dc.contributor.authorStahl, David A.
dc.contributor.authorWall, Judy D.
dc.contributor.authorHazen, Terry C.
dc.contributor.authorFields, Matthew W.
dc.contributor.authorArkin, Adam P.
dc.contributor.authorZhou, Jizhong
dc.identifier.citationZhou A, Hillesland KL , He, Schackwitz W, Qichao T, Zane GM, Qiao M, Qu Y, Stahl D, Wall J, Hazen T, Fields M, Arkin A, Zhou J, “Rapid Selective Sweep of Pre-Existing Polymorphisms and Slow Fixation of New Mutations in Experimental Evolution of Desulfovibrio Vulgaris.” ISME Journal 9, no. 11 (April 7, 2015): 2360–2372. doi:10.1038/ismej.2015.45.en_US
dc.description.abstractTo investigate the genetic basis of microbial evolutionary adaptation to salt (NaCl) stress, populations of Desulfovibrio vulgaris Hildenborough (DvH), a sulfate-reducing bacterium important for the biogeochemical cycling of sulfur, carbon and nitrogen, and potentially the bioremediation of toxic heavy metals and radionuclides, were propagated under salt stress or non-stress conditions for 1200 generations. Whole-genome sequencing revealed 11 mutations in salt stress-evolved clone ES9-11 and 14 mutations in non-stress-evolved clone EC3-10. Whole-population sequencing data suggested the rapid selective sweep of the pre-existing polymorphisms under salt stress within the first 100 generations and the slow fixation of new mutations. Population genotyping data demonstrated that the rapid selective sweep of pre-existing polymorphisms was common in salt stress-evolved populations. In contrast, the selection of pre-existing polymorphisms was largely random in EC populations. Consistently, at 100 generations, stress-evolved population ES9 showed improved salt tolerance, namely increased growth rate (2.0-fold), higher biomass yield (1.8-fold) and shorter lag phase (0.7-fold) under higher salinity conditions. The beneficial nature of several mutations was confirmed by site-directed mutagenesis. All four tested mutations contributed to the shortened lag phases under higher salinity condition. In particular, compared with the salt tolerance improvement in ES9-11, a mutation in a histidine kinase protein gene lytS contributed 27% of the growth rate increase and 23% of the biomass yield increase while a mutation in hypothetical gene DVU2472 contributed 24% of the biomass yield increase. Our results suggested that a few beneficial mutations could lead to dramatic improvements in salt tolerance.en_US
dc.description.sponsorshipENIGMA- Ecosystems and Networks Integrated with Genes and Molecular Assemblies a Scientific Focus Area Program at Lawrence Berkeley National Laboratory is based upon work supported by the US Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number (DE-AC02-05CH11231)en_US
dc.titleRapid selective sweep of pre-existing polymorphisms and slow fixation of new mutations in experimental evolution of Desulfovibrio vulgarisen_US
mus.citation.journaltitleISME Journalen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidFields, Matthew W.|0000-0001-9053-1849en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.