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dc.contributor.authorSkorupa, Dana J.
dc.contributor.authorCastenholz, R. W.
dc.contributor.authorMazurie, Aurélien J.
dc.contributor.authorCarey, Charles C.
dc.contributor.authorRosenzweig, F.
dc.contributor.authorMcDermott, Timothy R.
dc.identifier.citationSkorupa DJ, Castenholz RW, Mazurie A, Carey C, Rosenzweig F, McDermott TR, "In situ gene expression profiling of the thermoacidophilic alga Cyanidioschyzon in relation to visible and ultraviolet irradiance," Environmental Microbiology, June 2014 16(6): 1627–1641.en_US
dc.description.abstractUltraviolet and high-intensity visible radiation generate reactive intermediates that damage phototrophic microorganisms. In Yellowstone National Park, the thermoacidophilic alga Cyanidioschyzon exhibits an annual seasonal biomass fluctuation referred to as 'mat decline', where algal viability decreases as ultraviolet and visible irradiances increase during summer. We examined the role irradiance might play in mat decline using irradiance filters that uncouple ultraviolet and visible effects along with custom microarrays to study gene expression in situ. Of the 6,507 genes, 88% showed no response to ultraviolet or visible, implying that at the biomolecular level, these algae inhabit a chemostat-like environment and is consistent with the near constant aqueous chemistry measured. The remaining genes exhibited expression changes linked to ultraviolet exposure, to increased visible radiation, or to the apparent combined effects of ultraviolet and visible. Expression of DNA repetitive elements was synchronized, being repressed by visible but also influenced by ultraviolet. At highest irradiance levels, these algae reduced transcription of genes encoding functions involved with DNA replication, photosynthesis and cell cycle progression but exhibited an uptick in activities related to repairing DNA damage. This corroborates known physiological responses to ultraviolet and visible radiation, and leads us to provisionally conclude that mat decline is linked to photoinhibition.en_US
dc.description.sponsorshipU.S. National Science Foundation (MCB 0702212); Montana Agricultural Experiment Station (911310); U.S. National Science Foundation (MCB 0702177)en_US
dc.titleIn situ gene expression profiling of the thermoacidophilic alga C yanidioschyzon in relation to visible and ultraviolet irradianceen_US
mus.citation.journaltitleEnvironmental Microbiologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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