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dc.contributor.authorEustance, E. O.
dc.contributor.authorGardner, Robert D.
dc.contributor.authorMoll, Karen M.
dc.contributor.authorMenicucci, Joseph A. Jr.
dc.contributor.authorGerlach, Robin
dc.contributor.authorPeyton, Brent M.
dc.identifier.citationEustance E, Gardner RD, Moll KM, Menicucci J, Gerlach R, Peyton BM, "Growth, nitrogen utilization and biodiesel potential for two chlorophytes grown on ammonium, nitrate or urea," Journal of Applied Phycology 2013 25:1663–1677en_US
dc.description.abstractNitrogen removal from wastewater by algae provides the potential benefit of producing lipids for biodiesel and biomass for anaerobic digestion. Further, ammonium is the renewable form of nitrogen produced during anaerobic digestion and one of the main nitrogen sources associated with wastewater. The wastewater isolates Scenedesmus sp. 131 and Monoraphidium sp. 92 were grown with ammonium, nitrate, or urea in the presence of 5 % CO2, and ammonium and nitrate in the presence of air to optimize the growth and biofuel production of these chlorophytes. Results showed that growth on ammonium, in both 5 % CO2 and air, caused a significant decrease in pH during the exponential phase, causing growth inhibition due to the low buffering capacity of the medium. Therefore, biological buffers and pH controllers were utilized to prevent a decrease in pH. Growth on ammonium with pH control (synthetic buffers or KOH dosing) demonstrated that growth (rate and yield), biodiesel production, and ammonium utilization, similar to nitrate- and urea-amended treatments, can be achieved if sufficient CO2 is available. Since the use of buffers is economically limited to laboratory-scale experiments, chemical pH control could bridge the gap encountered in the scale-up to industrial processes.en_US
dc.titleGrowth, nitrogen utilization and biodiesel potential for two chlorophytes grown on ammonium, nitrate or ureaen_US
mus.citation.journaltitleJournal of Applied Phycologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidPeyton, Brent M.|0000-0003-0033-0651en_US

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