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dc.contributor.authorBhardwaj, C.
dc.contributor.authorCui, Y.
dc.contributor.authorHofstetter, T.
dc.contributor.authorLiu, S. Y.
dc.contributor.authorBernstein, Hans C.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorAhmed, M.
dc.contributor.authorHanley, L.
dc.date.accessioned2017-01-24T17:09:56Z
dc.date.available2017-01-24T17:09:56Z
dc.date.issued2013-09
dc.identifier.citationBhardwaj C, Cui Y, Hofstetter T, Liu SY, Bernstein HC, Carlson RP, Ahmed M, Hanley L, "Differentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectra," Analyst. 2013 Nov 21 138(22):6844-51.en_US
dc.identifier.issn0003-2654
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12424
dc.description.abstract7.87 to 10.5 eV vacuum ultraviolet (VUV) photon energies were used in laser desorption postionization mass spectrometry (LDPI-MS) to analyze biofilms comprised of binary cultures of interacting microorganisms. The effect of photon energy was examined using both tunable synchrotron and laser sources of VUV radiation. Principal components analysis (PCA) was applied to the MS data to differentiate species in Escherichia coli–Saccharomyces cerevisiae coculture biofilms. PCA of LDPI-MS also differentiated individual E. coli strains in a biofilm comprised of two interacting gene deletion strains, even though these strains differed from the wild type K-12 strain by no more than four gene deletions each out of approximately 2000 genes. PCA treatment of 7.87 eV LDPI-MS data separated the E. coli strains into three distinct groups, two “pure†groups, and a mixed region. Furthermore, the “pure†regions of the E. coli cocultures showed greater variance by PCA at 7.87 eV photon energies compared to 10.5 eV radiation. This is consistent with the expectation that the 7.87 eV photoionization selects a subset of low ionization energy analytes while 10.5 eV is more inclusive, detecting a wider range of analytes. These two VUV photon energies therefore give different spreads via PCA and their respective use in LDPI-MS constitute an additional experimental parameter to differentiate strains and species.en_US
dc.titleDifferentiation of microbial species and strains in coculture biofilms by multivariate analysis of laser desorption postionization mass spectraen_US
dc.typeArticleen_US
mus.citation.extentfirstpage6844en_US
mus.citation.extentlastpage6851en_US
mus.citation.issue22en_US
mus.citation.journaltitleAnalysten_US
mus.citation.volume138en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1039/c3an01389hen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US


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