Show simple item record

dc.contributor.authorBernstein, H. C.
dc.contributor.authorBeam, Jacob P.
dc.contributor.authorKozubal, Mark A.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorInskeep, William P.
dc.date.accessioned2017-01-27T21:19:32Z
dc.date.available2017-01-27T21:19:32Z
dc.date.issued2013-03
dc.identifier.citationBernstein HC, Beam JP, Kozubal MA, Carlson RP, Inskeep WP, "In situ analysis of oxygen consumption and diffusive transport in 1 high-temperature acidic iron-oxide microbial mats," Environ Microbiol 2013 15(8):2360-2370en_US
dc.identifier.issn1462-2912
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12451
dc.description.abstractThe role of dissolved oxygen as a principal electron acceptor for microbial metabolism was investigated within Fe(III)-oxide microbial mats that form in acidic geothermal springs of Yellowstone National Park (USA). Specific goals of the study were to measure and model dissolved oxygen profiles within high temperature (65–75°C) acidic (pH = 2.7–3.8) Fe(III)-oxide microbial mats, and correlate the abundance of aerobic, iron-oxidizing Metallosphaera yellowstonensis organisms and mRNA gene expression levels to Fe(II)-oxidizing habitats shown to consume oxygen. In situ oxygen microprofiles were obtained perpendicular to the direction of convective flow across the aqueous phase/Fe(III)-oxide microbial mat interface using oxygen microsensors. Dissolved oxygen concentrations dropped from ~ 50–60 µM in the bulkfluid/ mat surface to below detection (< 0.3 µM) at a depth of ~ 700 µm (~ 10% of the total mat depth). Net areal oxygen fluxes into the microbial mats were estimated to range from 1.4–1.6 ¥ 10-4 µmol cm-2 s-1. Dimensionless parameters were used to model dissolved oxygen profiles and establish that mass transfer rates limit the oxygen consumption. A zone of higher dissolved oxygen at the mat surface promotes Fe(III)-oxide biomineralization, which was supported using molecular analysis of Metallosphaera yellowstonensis 16S rRNA gene copy numbers and mRNA expression of haem Cu oxidases (FoxA) associated with Fe(II)-oxidation.en_US
dc.titleIn situ analysis of oxygen consumption and diffusive transport in 1 high-temperature acidic iron-oxide microbial matsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2360en_US
mus.citation.extentlastpage2370en_US
mus.citation.issue8en_US
mus.citation.journaltitleEnvironmental Microbiologyen_US
mus.citation.volume15en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1111/1462-2920.12109en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentEcology.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record