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dc.contributor.authorValenzuela, Jacob J.
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorGerlach, Robin
dc.contributor.authorCooksey, Keith E.
dc.contributor.authorPeyton, Brent M.
dc.contributor.authorBothner, Brian
dc.contributor.authorFields, Matthew W.
dc.identifier.citationValenzuela J, Carlson RP, Gerlach R, Cooksey KE, Peyton BM, Bothner B, Fields MW, "Nutrient resupplementation arrests bio-oil accumulation in Phaeodactylum tricornutum," Applied Microbiology and Biotechnology, August 2013 97(15):7049–7059en_US
dc.description.abstractPhaeodactylum tricornutum is a marine diatom in the class Bacillariophyceae and is important ecologically and industrially with regards to ocean primary production and lipid accumulation for biofuel production, respectively. Triacylglyceride (TAG) accumulation has been reported in P. tricornutum under different nutrient stresses, and our results show that lipid accumulation can occur with nitrate or phosphate depletion. However, greater lipid accumulation was observed when both nutrients were depleted as observed using a Nile Red assay and fatty acid methyl ester (FAME) profiles. Nitrate depletion had a greater effect on lipid accumulation than phosphate depletion. Lipid accumulation in P. tricornutum was arrested upon resupplementation with the depleted nutrient. Cells depleted of nitrogen showed a distinct shift from a lipid accumulation mode to cellular growth post resupplementation with nitrate, as observed through increased cell numbers and consumption of accumulated lipid. Phosphate depletion caused lipid accumulation that was arrested upon phosphate resupplementation. The cessation of lipid accumulation was followed by lipid consumption without an increase in cell numbers. Cells depleted in both nitrate and phosphate displayed cell growth upon the addition of both nitrate and phosphate and had the largest observed lipid consumption upon resupplementation. These results indicate that phosphate resupplementation can shut down lipid accumulation but does not cause cells to shift into cellular growth, unlike nitrate resupplementation. These data suggest that nutrient resupplementation will arrest lipid accumulation and that switching between cellular growth and lipid accumulation can be regulated upon the availability of nitrogen and phosphorus.en_US
dc.rightsCC BY 4.0en_US
dc.titleNutrient resupplementation arrests bio-oil accumulation in Phaeodactylum tricornutumen_US
mus.citation.journaltitleApplied Microbiology and Biotechnologyen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentAgricultural Engineering.en_US
mus.relation.departmentBiological Sciences.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidBothner, Brian|0000-0003-1295-9609en_US
mus.contributor.orcidFields, Matthew W.|0000-0001-9053-1849en_US
mus.contributor.orcidPeyton, Brent M.|0000-0003-0033-0651en_US

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