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dc.contributor.authorPines, R. M.
dc.contributor.authorTomasino, S. F.
dc.contributor.authorCottrill, M. P.
dc.contributor.authorHamilton, G. C.
dc.contributor.authorParker, Albert E.
dc.date.accessioned2017-01-27T23:38:10Z
dc.date.available2017-01-27T23:38:10Z
dc.date.issued2013-06
dc.identifier.citationPines RM, Tomasino SF, Cottrill MP, Hamilton GC, Parker AE, "Procedural revision to the AOAC germicidal spray products as disinfectants test method: Establishment of minimum and maximum log density values for test microbes on inoculated carriers," Journal of AOAC International, May-June 2013 96(3): 567-572.en_US
dc.identifier.issn1060-3271
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12464
dc.description.abstractThe AOAC Germicidal Spray Products as Disinfectants test method (AOAC Official Method 961.02) is used to measure the efficacy of spray products on hard inanimate surfaces; however, the method does not provide procedures to determine the population of the test microbe on inoculated glass slide carriers (e.g., carrier counts reported as CFU/carrier). Without a method to measure and monitor carrier counts, the associated efficacy data may not be reliable and repeatable. This report provides a standardized procedure to address this issue and, based on carrier count data collected by four laboratories from 2000 to 2010, proposes a specific range for the mean log density per carrier as a requirement. Laboratory-based carrier count data were collected concurrently with 116 Method 961.02 efficacy tests conducted on spray products bearing claims against Pseudomonas aeruginosa and Staphylococcus aureus. For many of the tests a soil load (SL) was added to the inoculum (as specified on the product label claim). Six carriers were assayed per test for a total of 696 carriers. All but two of the 116 mean log densities were at least 5.0 (a geometric mean of 1.0 × 105 CFU/carrier). Across the four combinations of microbes and SL treatments, the mean TestLD (mean log density across all enumerated carriers in a test) ranged from approximately 6.0 (a geometric mean of 0.9 × 106 CFU/carrier) to 6.3 (a geometric mean of 2.0 × 106 CFU/carrier). Across all microbes and SL treatments, the mean log density (±SEM) was 6.2 (±0.07) per carrier (a geometric mean of 1.5 × 106 CFU/carrier). The mean log density for six carriers per test showed good repeatability (0.32) and reproducibility (0.34). The proposed requirement for S. aureus tests and P. aeruginosa tests is a mean log density (across six carriers) between 5.0 and 6.5. A separate 2009 study at three laboratories was conducted to evaluate the persistence of P. aeruginosa, S. aureus, and Salmonella enterica on glass carriers. Based on the persistence data, a 2 h use period is proposed for using the inoculated carriers post drying. The persistence data set was also used to assess the carrier counts for S. enterica. The carrier counts were approximately one log lower for S. enterica compared to S. aureus and P. aeruginosa; a range of 4.0 to 5.5 logs is proposed as a requirement for S. enterica tests.en_US
dc.titleProcedural revision to the AOAC germicidal spray products as disinfectants test method: Establishment of minimum and maximum log density values for test microbes on inoculated carriersen_US
dc.typeArticleen_US
mus.citation.extentfirstpage567en_US
mus.citation.extentlastpage572en_US
mus.citation.issue3en_US
mus.citation.journaltitleJournal of AOAC Internationalen_US
mus.citation.volume93en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.5740/jaoacint.12-406en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentHealth & Human Development.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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