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dc.contributor.authorSlaughter, D. C.
dc.contributor.authorMacur, Richard E.
dc.contributor.authorInskeep, William P.
dc.date.accessioned2017-02-02T23:16:36Z
dc.date.available2017-02-02T23:16:36Z
dc.date.issued2012-03
dc.identifier.citationSlaughter DC, Macur RE, Inskeep WP, "Inhibition of microbial arsenate reduction by phosphate," Microbiological Research 2012 167(3):151–156en_US
dc.identifier.issn0944-5013
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12549
dc.description.abstractThe ratio of arsenite (AsIII) to arsenate (AsV) in soils and natural waters is often controlled by the activity of As-transforming microorganisms. Phosphate is a chemical analog to AsV and, consequently, may competitively inhibit microbial uptake and enzymatic binding of AsV, thus preventing its reduction to the more toxic, mobile, and bioavailable form – AsIII. Five As-transforming bacteria isolated either from As-treated soil columns or from As-impacted soils were used to evaluate the effects of phosphate on AsV reduction and AsIII oxidation. Cultures were initially spiked with various P:As ratios, incubated for approximately 48 h, and analyzed periodically for AsV and AsIII concentration. Arsenate reduction was inhibited at high P:As ratios and completely suppressed at elevated levels of phosphate (500 and 1000μM; P inhibition constant (Ki)~20–100 μM). While high P:As ratios effectively shut down microbial AsV reduction, the expression of the arsenate reductase gene (arsC) was not inhibited under these conditions in the AsVreducing isolate, Agrobacterium tumefaciens str. 5B. Further, high phosphate ameliorated AsV-induced cell growth inhibition caused by high (1 mM) As pressure. These results indicate that phosphate may inhibit AsV reduction by impeding AsV uptake by the cell via phosphate transport systems or by competitively binding to the active site of ArsC.en_US
dc.titleInhibition of microbial arsenate reduction by phosphateen_US
dc.typeArticleen_US
mus.citation.extentfirstpage151en_US
mus.citation.extentlastpage156en_US
mus.citation.issue3en_US
mus.citation.journaltitleMicrobiological Researchen_US
mus.citation.volume167en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1016/j.micres.2011.05.007en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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