Show simple item record

dc.contributor.authorSlaughter, D. C.
dc.contributor.authorMacur, Richard E.
dc.contributor.authorInskeep, William P.
dc.identifier.citationSlaughter DC, Macur RE, Inskeep WP, "Inhibition of microbial arsenate reduction by phosphate," Microbiological Research 2012 167(3):151–156en_US
dc.description.abstractThe ratio of arsenite (AsIII) to arsenate (AsV) in soils and natural waters is often controlled by the activity of As-transforming microorganisms. Phosphate is a chemical analog to AsV and, consequently, may competitively inhibit microbial uptake and enzymatic binding of AsV, thus preventing its reduction to the more toxic, mobile, and bioavailable form – AsIII. Five As-transforming bacteria isolated either from As-treated soil columns or from As-impacted soils were used to evaluate the effects of phosphate on AsV reduction and AsIII oxidation. Cultures were initially spiked with various P:As ratios, incubated for approximately 48 h, and analyzed periodically for AsV and AsIII concentration. Arsenate reduction was inhibited at high P:As ratios and completely suppressed at elevated levels of phosphate (500 and 1000μM; P inhibition constant (Ki)~20–100 μM). While high P:As ratios effectively shut down microbial AsV reduction, the expression of the arsenate reductase gene (arsC) was not inhibited under these conditions in the AsVreducing isolate, Agrobacterium tumefaciens str. 5B. Further, high phosphate ameliorated AsV-induced cell growth inhibition caused by high (1 mM) As pressure. These results indicate that phosphate may inhibit AsV reduction by impeding AsV uptake by the cell via phosphate transport systems or by competitively binding to the active site of ArsC.en_US
dc.titleInhibition of microbial arsenate reduction by phosphateen_US
mus.citation.journaltitleMicrobiological Researchen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.