A modified CDC biofilm reactor to produce mature biofilms on the surface of PEEK membranes for an in vivo animal model application
Williams, D. L.
Woodbury, K. L.
Haymond, B. S.
Parker, Albert E.
Bloebaum, R. D.
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Biofilm-related infections have become a major clinical concern. Typically, animal models that involve inoculation with planktonic bacteria have been used to create positive infection signals and examine antimicrobial strategies for eradicating or preventing biofilm-related infection. However, it is estimated that 99.9%of bacteria in nature dwell in established biofilms. As such, openwounds have significant potential to become contaminatedwith bacteria that reside in a well-established biofilm. In this study, a modified CDC biofilm reactor was developed to repeatably grow mature biofilms of Staphylococcus aureus on the surface of polyetheretherketone (PEEK) membranes for inoculation in a future animal model of orthopaedic implant biofilm-related infection. Results indicated that uniform, mature biofilms repeatably grew on the surface of the PEEK membranes.
Williams DL, Woodbury KL, Haymond BS, Parker AE, Bloebaum RD, "A modified CDC biofilm reactor to produce mature biofilms on the surface of PEEK membranes for an in vivo animal model application," Current Microbiology, March 2011 62(6)1657-1663