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dc.contributor.authorVanEngelen, Michael R.
dc.contributor.authorSzilagyi, Robert K.
dc.contributor.authorGerlach, Robin
dc.contributor.authorLee, Brady D.
dc.contributor.authorApel, William A.
dc.contributor.authorPeyton, Brent M.
dc.identifier.citationVanEngelen MR, Szilagyi RK, Gerlach R, Lee BD, Apel WA, Peyton BM, "Uranium exerts acute toxicity by binding to pyrroloquinoline quinone cofactor," Environmental Science & Technology, 2011 45(3):937–942en_US
dc.description.abstractUranium as an environmental contaminant has been shown to be toxic to eukaryotes and prokaryotes; however, no specific mechanisms of uranium toxicity have been proposed so far. Here a combination of in vivo, in vitro, and in silico studies are presented describing direct inhibition of pyrroloquinoline quinone (PQQ)-dependent growth and metabolism by uranyl cations. Electrospray-ionization mass spectroscopy, UV-vis optical spectroscopy, competitive Ca2+/uranyl binding studies, relevant crystal structures, and molecular modeling unequivocally indicate the preferred binding of uranyl simultaneously to the carboxyl oxygen, pyridine nitrogen, and quinone oxygen of the PQQmolecule. The observed toxicity patterns are consistent with the biotic ligand model of acute metal toxicity. In addition to the environmental implications, this work represents the first proposed molecular mechanism of uranium toxicity in bacteria, and has relevance for uranium toxicity in many living systems.en_US
dc.titleUranium exerts acute toxicity by binding to pyrroloquinoline quinone cofactoren_US
mus.citation.journaltitleEnvironmental Science & Technologyen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidPeyton, Brent M.|0000-0003-0033-0651en_US

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