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dc.contributor.authorAgostinho, Alessandra
dc.contributor.authorHartman, A.
dc.contributor.authorLipp, C.
dc.contributor.authorParker, Albert E.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorJames, Garth A.
dc.date.accessioned2017-02-07T18:41:47Z
dc.date.available2017-02-07T18:41:47Z
dc.date.issued2011-09
dc.identifier.citationAgostinho AM, Hartman A, Lipp C, Parker AE, Stewart PS, James GA, "An in vitro model for the growth and analysis of chronic wound MRSA biofilms," Journal of Applied Microbiology 2011 111(5):1275–1282en_US
dc.identifier.issn1364-5072
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12582
dc.description.abstractAims: To develop an in vitro model (Colony/drip-flow reactor – C/DFR) for the growth and analysis of methicillin-resistant Staphylococcus aureus (MRSA) biofilms. Methods and Results: Using the C/DFR model, biofilms were grown on the top of polycarbonate filter membranes inoculated with a clinical isolate of MRSA, placed on absorbent pads in the DFR and harvested after 72 h. The biofilms varied from 256 to 308 µm in thickness with a repeatability standard deviation of 0·22. Testing of antimicrobial agents was also performed where C/DFR biofilms were grown in parallel with conventional colony biofilms. A saline solution (control), 1% silver sulfadiazine solution, and 0·25% Dakin’s solution were used to treat the biofilms for 15 min. Microscopic evaluation of biofilm morphology and thickness was conducted. The Dakins solution in both models produced statistically significantly higher log reductions than silver sulfadiazine treatment. Conclusions: The C/DFR biofilms were thick and repeatable and exhibited higher resistance to Dakins solution than the treated colony biofilms. Significance and Impact of the Study: The C/DFR can be used as a tool for examining complex biofilm physiology as well as for performing comparative experiments that test wound care products and novel antimicrobials.en_US
dc.titleAn in vitro model for the growth and analysis of chronic wound MRSA biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1275en_US
mus.citation.extentlastpage1282en_US
mus.citation.issue5en_US
mus.citation.journaltitleJournal of Applied Microbiologyen_US
mus.citation.volume111en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryHealth & Medical Sciencesen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1111/j.1365-2672.2011.05138.xen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Education, Health & Human Developmenten_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentHealth & Human Development.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage2en_US


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