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dc.contributor.authorGonzalez-Ballester, D.
dc.contributor.authorPootakham, W.
dc.contributor.authorMus, Florence
dc.contributor.authorYang, Wenqiang
dc.contributor.authorCatalanotti, C.
dc.contributor.authorMagneschi, L.
dc.contributor.authorde Montaigu, A.
dc.contributor.authorHiguera, J. J.
dc.contributor.authorPrior, M.
dc.contributor.authorGalvan, A.
dc.contributor.authorFernandez, E.
dc.contributor.authorGrossman, A. R.
dc.identifier.citationGonzalez-Ballester D, Pootakham W, Mus F, Yang W, Catalanotti C, Magneschi L, de Montaigu A, Higuera JJ, Prior M, Galván A, Fernandez E, Grossman AR, "Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants," Plant Methods 2011 7(1):24en_US
dc.description.abstractA method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene within specific genes of interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature of the marker DNA used (PCR-amplified fragment containing the plasmid-free marker versus entire linearized plasmid with the marker) and in the strategies used to maintain and store transformants.en_US
dc.rightsCC BY 4.0en_US
dc.titleReverse genetics in Chlamydomonas: a platform for isolating insertional mutantsen_US
mus.citation.journaltitlePlant Methodsen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidMus, Florence|0000-0002-1655-1267en_US

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