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dc.contributor.authorSchultz, Logan N.
dc.contributor.authorPitts, Betsey
dc.contributor.authorMitchell, Andrew C.
dc.contributor.authorCunningham, Alfred B.
dc.contributor.authorGerlach, Robin
dc.date.accessioned2017-02-13T17:03:18Z
dc.date.available2017-02-13T17:03:18Z
dc.date.issued2011
dc.identifier.citationSchultz L, Pitts B, Mitchell AC, Cunningham AB, Gerlach R, "Imaging biologically induced mineralization in fully hydrated flow systems," Microscopy Today 2011 19(5):12-15en_US
dc.identifier.issn1551-9295
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12598
dc.description.abstractA number of proposed technologies involve the controlled implementation of biologically induced carbonate mineral precipitation in the geologic subsurface. Examples include the enhancement of soil stability [1], immobilization of groundwater contaminants such as strontium and uranium [2], and the enhancement of oil recovery and geologic carbon sequestration via controlled permeability reduction [3]. The most significant challenge in these technologies remains to identify and better understand an industrially, environmentally, and economically viable carbonate precipitation route.One of the most promising routes is ureolytic biomineralization, because of the ample availability of urea and the controllable reaction rate. In this process, ureolytic bacteria hydrolyze urea, leading to an increase in pH. In the presence of calcium, this process favors the formation of solid calcium carbonate, as illustrated in the following equations:CO(NH2)2 + H2O → NH2COOH + NH3→ 2 NH3 + CO2 (Urea hydrolysis) (1)2 NH3 + 2 H2O ↔ 2NH4+ + 2OH– (pH increase) (2)CO2 + 2 OH– ↔ CO32– + H2O(Carbonate ion formation) (3)CO32– + Ca2+ ↔ CaCO3 (solid)(Precipitation is favored at high pH) (4)This process relies on molecular-level chemical and biological processes that must be better understood for large-scale implementation.Researchers at the Center for Biofilm Engineering at Montana State University (USA) and Aberystwyth University (UK) have conducted several biomineralization experiments in simulated porous media reactors. Microscopy has proven to be one of the most useful analytical tools in these studies, providing the ability to non-invasively visualize, differentiate, and quantify the various components, including the cells, cell matrix, and mineral precipitates. Because of the possibility of real-time observation and the lack of dehydration artifacts, microscopy has been tremendously useful for elucidating the temporal and spatial relationships of these components.en_US
dc.titleImaging biologically induced mineralization in fully hydrated flow systemsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage12en_US
mus.citation.extentlastpage15en_US
mus.citation.issue5en_US
mus.citation.journaltitleMicroscopy Todayen_US
mus.citation.volume19en_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1017/s1551929511000848en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentEcology.en_US
mus.relation.departmentEnvironmental Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupZero Emissions Research and Technology (ZERT)
mus.data.thumbpage3en_US


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