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dc.contributor.authorHe, Q.
dc.contributor.authorHe, Zhili
dc.contributor.authorJoyner, D. C.
dc.contributor.authorJoachimiak, M. P.
dc.contributor.authorPrice, M. N.
dc.contributor.authorYang, Zamin K.
dc.contributor.authorYen, Huei-Che B.
dc.contributor.authorHemme, C. L.
dc.contributor.authorChen, W.
dc.contributor.authorFields, Matthew W.
dc.contributor.authorStahl, David A.
dc.contributor.authorKeasling, J. D.
dc.contributor.authorKeller, M.
dc.contributor.authorArkin, Adam P.
dc.contributor.authorHazen, Terry C.
dc.contributor.authorWall, Judy D.
dc.contributor.authorZhou, Jizhong
dc.identifier.citationHe Q, He Z, Joyner DC, Joachimiak M, Price MN, Yang ZK, Yen H-C B, Hemme CL, Chen W, Fields MW, Stahl DA, Keasling JD, Keller M, Arkin AP, Hazen TC, Wall JD, Zhou J, "Impact of elevated nitrate on sulfate-reducing bacteria: A comparative study of Desulfovibrio vulgaris," ISME J 2010 epublished May 6en_US
dc.description.abstractSulfate-reducing bacteria have been extensively studied for their potential in heavy-metal bioremediation. However, the occurrence of elevated nitrate in contaminated environments has been shown to inhibit sulfate reduction activity. Although the inhibition has been suggested to result from the competition with nitrate-reducing bacteria, the possibility of direct inhibition of sulfate reducers by elevated nitrate needs to be explored. Using Desulfovibrio vulgaris as a model sulfate-reducing bacterium, functional genomics analysis reveals that osmotic stress contributed to growth inhibition by nitrate as shown by the upregulation of the glycine/betaine transporter genes and the relief of nitrate inhibition by osmoprotectants. The observation that significant growth inhibition was effected by 70mM NaNO3 but not by 70mM NaCl suggests the presence of inhibitory mechanisms in addition to osmotic stress. The differential expression of genes characteristic of nitrite stress responses, such as the hybrid cluster protein gene, under nitrate stress condition further indicates that nitrate stress response by D. vulgaris was linked to components of both osmotic and nitrite stress responses. The involvement of the oxidative stress response pathway, however, might be the result of a more general stress response. Given the low similarities between the response profiles to nitrate and other stresses, less-defined stress response pathways could also be important in nitrate stress, which might involve the shift in energy metabolism. The involvement of nitrite stress response upon exposure to nitrate may provide detoxification mechanisms for nitrite, which is inhibitory to sulfate-reducing bacteria, produced by microbial nitrate reduction as a metabolic intermediate and may enhance the survival of sulfate-reducing bacteria in environments with elevated nitrate level.en_US
dc.titleImpact of elevated nitrate on sulfate-reducing bacteria: A comparative study of Desulfovibrio vulgarisen_US
mus.citation.journaltitleThe ISME Journalen_US
mus.identifier.categoryChemical & Material Sciencesen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Letters & Scienceen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentChemistry & Biochemistry.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidFields, Matthew W.|0000-0001-9053-1849en_US

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