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dc.contributor.authorArcher-Zhang, Christian Chunzi
dc.contributor.authorFoster, Warren B.
dc.contributor.authorDowney, Ryan D.
dc.contributor.authorArrasmith, Christopher L.
dc.contributor.authorDickensheets, David L.
dc.identifier.citationArcher-Zhang, Christian Chunzi, Warren B. Foster, Ryan D. Downey, Christopher L. Arrasmith, and David L. Dickensheets. "Dynamic performance of microelectromechanical systems deformable mirrors for use in an active/adaptive two-photon microscope." Journal of Biomedical Optics 21, no. 12 (December 2016). DOI:
dc.description.abstractActive optics such as deformable mirrors can be used to control both focal depth and aberrations during scanning laser microscopy. If the focal depth can be changed dynamically during scanning, then imaging of oblique surfaces becomes possible. If aberrations can be corrected dynamically during scanning, an image can be optimized throughout the field of view. Here, we characterize the speed and dynamic precision of a Boston Micromachines Corporation Multi-DM 140 element aberration correction mirror and a Revibro Optics 4-zone focus control mirror to assess suitability for use in an active and adaptive two-photon microscope. Tests for the multi-DM include both step response and sinusoidal frequency sweeps of specific Zernike modes (defocus, spherical aberration, coma, astigmatism, and trefoil). We find wavefront error settling times for mode amplitude steps as large as 400 nm to be less than 52 mu s, with 3 dB frequencies ranging from 6.5 to 10 kHz. The Revibro Optics mirror was tested for step response only, with wavefront error settling time less than 80 mu s for defocus steps up to 3000 nm, and less than 45 mu s for spherical aberration steps up to 600 nm. These response speeds are sufficient for intrascan correction at scan rates typical of two-photon microscopy. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.en_US
dc.description.sponsorshipNational Science Foundation (1338133); NSF National Nanotechnology Coordinated Infrastructure Program (1542210)en_US
dc.titleDynamic performance of microelectromechanical systems deformable mirrors for use in an active/adaptive two-photon microscopeen_US
mus.citation.journaltitleJournal of Biomedical Opticsen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentElectrical & Computer Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US

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