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dc.contributor.authorNegri, Jackson L.
dc.date.accessioned2017-06-02T22:35:42Z
dc.date.available2017-06-02T22:35:42Z
dc.date.issued2017-04
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/12929
dc.description.abstractMicroRNA are small endogenous noncoding molecules of only 19-23 nucleotides in length. These tiny molecules play an important role in many processes in the human. Due to their limited length, miRNA sequences are very difficult to reliably detect and quantify. One possible detection method, exponential amplification reaction (EXPAR), is already being used for miRNA detection, viral DNA detection, and genomic DNA detection. The amplification process is specific and non-specific, the non-specific amplification makes it difficult to study the molecule of interest. More specific miRNA amplification would lead to a greater understanding of miRNA function in pathological processes would enable earlier disease diagnoses and potentially provide new therapies. On site diagnosis of miRNA for disease detection would be a breakthrough for biomedical research. One way to increase the specific rate of miRNA amplification is to use a stem loop template. The stem loop will help prevent the non-specific amplification of the molecule low, until the specific trigger molecule is present to initiate amplification. Altering enzyme, loop, dNTP, and magnesium concentrations were done to determine the best reaction environment. A matlab model was also created to model the reaction and determine how changing different concentrations affected the reaction.en_US
dc.language.isoen_USen_US
dc.publisherMontana State Universityen_US
dc.titleDecreasing the ratio non-specific to specicif miRNA amplification using a stem loop templateen_US
dc.typePresentationen_US
mus.citation.conferenceStudent Research Celebrationen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage1en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US


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