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dc.contributor.authorŞengör, S. Sevinç
dc.contributor.authorSingh, Gursharan
dc.contributor.authorDohnalkova, Alice
dc.contributor.authorSpycher, Nicolas
dc.contributor.authorGinn, Timothy R.
dc.contributor.authorPeyton, Brent M.
dc.contributor.authorSani, Rajesh K.
dc.date.accessioned2017-06-13T20:22:55Z
dc.date.available2017-06-13T20:22:55Z
dc.date.issued2016-12
dc.identifier.citationŞengör SS, Singh G, Dohnalkova A, Spycher N, Ginn TR, Peyton BM, Sani RK, “Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20,” BioMetals. 2016 December;29(6):965-980.en_US
dc.identifier.issn0966-0844
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13061
dc.description.abstractThis study investigates the impact of specific environmental conditions on the formation of colloidal U(IV) nanoparticles by the sulfate reducing bacteria (SRB, Desulfovibrio alaskensis G20). The reduction of soluble U(VI) to less soluble U(IV) was quantitatively investigated under growth and non-growth conditions in bicarbonate or 1,4-piperazinediethanesulfonic acid (PIPES) buffered environments. The results showed that under non-growth conditions, the majority of the reduced U nanoparticles aggregated and precipitated out of solution. High resolution transmission electron microscopy revealed that only a very small fraction of cells had reduced U precipitates in the periplasmic spaces in the presence of PIPES buffer, whereas in the presence of bicarbonate buffer, reduced U was also observed in the cytoplasm with greater aggregation of biogenic U(IV) particles at higher initial U(VI) concentrations. The same experiments were repeated under growth conditions using two different electron donors (lactate and pyruvate) and three electron acceptors (sulfate, fumarate, and thiosulfate). In contrast to the results of the non-growth experiments, even after 0.2 μm filtration, the majority of biogenic U(IV) remained in the aqueous phase resulting in potentially mobile biogenic U(IV) nanoparticles. Size fractionation results showed that U(IV) aggregates were between 18 and 200 nm in diameter, and thus could be very mobile. The findings of this study are helpful to assess the size and potential mobility of reduced U nanoparticles under different environmental conditions, and would provide insights on their potential impact affecting U(VI) bioremediation efforts at subsurface contaminated sites.en_US
dc.titleImpact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20en_US
dc.typeArticleen_US
mus.citation.extentfirstpage965en_US
mus.citation.extentlastpage980en_US
mus.citation.issue6en_US
mus.citation.journaltitleBioMetalsen_US
mus.citation.volume29en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s10534-016-9969-6en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupThermal Biology Institute.en_US
mus.data.thumbpage11en_US
mus.contributor.orcidPeyton, Brent M.|0000-0003-0033-0651en_US


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