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dc.contributor.authorWang, Hong
dc.contributor.authorBédard, Emilie
dc.contributor.authorPrévost, Michèle
dc.contributor.authorCamper, Anne K.
dc.contributor.authorHill, Vincent R.
dc.contributor.authorPruden, Amy
dc.date.accessioned2017-06-19T16:22:48Z
dc.date.available2017-06-19T16:22:48Z
dc.date.issued2017-03
dc.identifier.citationWang H, Bédard E, Prévost M, Camper AK, Hill VR, Pruden A, “Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review,” Water Research, 2017 Mar 25;117:68-86. doi: 10.1016/j.watres.2017.03.046.en_US
dc.identifier.issn0043-1354
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13073
dc.description.abstractOpportunistic premise (i.e., building) plumbing pathogens (OPPPs, e.g., Legionella pneumophila, Mycobacterium avium complex, Pseudomonas aeruginosa, Acanthamoeba, and Naegleria fowleri) are a significant and growing source of disease. Because OPPPs establish and grow as part of the native drinking water microbiota, they do not correspond to fecal indicators, presenting a major challenge to standard drinking water monitoring practices. Further, different OPPPs present distinct requirements for sampling, preservation, and analysis, creating an impediment to their parallel detection. The aim of this critical review is to evaluate the state of the science of monitoring OPPPs and identify a path forward for their parallel detection and quantification in a manner commensurate with the need for reliable data that is informative to risk assessment and mitigation. Water and biofilm sampling procedures, as well as factors influencing sample representativeness and detection sensitivity, are critically evaluated with respect to the five representative bacterial and amoebal OPPPs noted above. Available culturing and molecular approaches are discussed in terms of their advantages, limitations, and applicability. Knowledge gaps and research needs towards standardized approaches are identified.en_US
dc.titleMethodological approaches for monitoring opportunistic pathogens in premise plumbing: A reviewen_US
dc.typeArticleen_US
mus.citation.extentfirstpage68en_US
mus.citation.extentlastpage86en_US
mus.citation.journaltitleWater Researchen_US
mus.citation.volume117en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1016/j.watres.2017.03.046en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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