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dc.contributor.authorZhang, Ping
dc.contributor.authorHe, Zhili
dc.contributor.authorVan Nostrand, Joy D.
dc.contributor.authorQin, Yujia
dc.contributor.authorDeng, Ye
dc.contributor.authorWu, Liyou
dc.contributor.authorTu, Qichao
dc.contributor.authorWang, Jianjun
dc.contributor.authorSchadt, Christopher W.
dc.contributor.authorFields, Matthew W.
dc.contributor.authorHazen, Terry C.
dc.contributor.authorArkin, Adam P.
dc.contributor.authorStahl, David A.
dc.contributor.authorZhou, Jizhong
dc.date.accessioned2017-06-19T16:23:42Z
dc.date.available2017-06-19T16:23:42Z
dc.date.issued2017-04
dc.identifier.citationZhang P, He Z, Van Nostrand JD, Qin Y, Deng Y, Wu L, Tu Q, Wang J, Schadt CW, Fields MW, Hazen TC, Arkin AP, Stahl DA, Zhou J, “Dynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquifer,” Environmental Science & Technology. 2017 April 4;51(7):3609-3620. doi:10.1021/acs.est.6b02980.en_US
dc.identifier.issn0013-936X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13076
dc.description.abstractTo further understand the diversity and dynamics of SRB in response to substrate amendment, we sequenced genes coding for the dissimilatory sulfite reductase (dsrA) in groundwater samples collected after an emulsified vegetable oil (EVO) amendment, which sustained U(VI)-reducing conditions for one year in a fast-flowing aquifer. EVO amendment significantly altered the composition of groundwater SRB communities. Sequences having no closely related-described species dominated (80%) the indigenous SRB communities in nonamended wells. After EVO amendment, Desulfococcus, Desulfobacterium, and Desulfovibrio, known for long-chain-fatty-acid, short-chain-fatty-acid and H2 oxidation and U(VI) reduction, became dominant accounting for 7 ± 2%, 21 ± 8%, and 55 ± 8% of the SRB communities, respectively. Succession of these SRB at different bioactivity stages based on redox substrates/products (acetate, SO4–2, U(VI), NO3–, Fe(II), and Mn(II)) was observed. Desulfovibrio and Desulfococcus dominated SRB communities at 4–31 days, whereas Desulfobacterium became dominant at 80–140 days. By the end of the experiment (day 269), the abundance of these SRB decreased but the overall diversity of groundwater SRB was still higher than non-EVO controls. Up to 62% of the SRB community changes could be explained by groundwater geochemical variables, including those redox substrates/products. A significant (P < 0.001) correlation was observed between groundwater U(VI) concentrations and Desulfovibrio abundance. Our results showed that the members of SRB and their dynamics were correlated significantly with slow EVO biodegradation, electron donor production and maintenance of U(VI)-reducing conditions in the aquifer.en_US
dc.titleDynamic Succession of Groundwater Sulfate-Reducing Communities during Prolonged Reduction of Uranium in a Contaminated Aquiferen_US
dc.typeArticleen_US
mus.citation.extentfirstpage3609en_US
mus.citation.extentlastpage3620en_US
mus.citation.issue7en_US
mus.citation.journaltitleEnvironmental Science & Technologyen_US
mus.citation.volume51en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1021/acs.est.6b02980en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US
mus.contributor.orcidFields, Matthew W.|0000-0001-9053-1849en_US


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