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dc.contributor.authorSuci, Peter A.
dc.contributor.authorKang, Sebyung
dc.contributor.authorGmür, Rudolf
dc.contributor.authorDouglas, Trevor
dc.contributor.authorYoung, Mark J.
dc.date.accessioned2017-06-21T14:06:25Z
dc.date.available2017-06-21T14:06:25Z
dc.date.issued2010-04
dc.identifier.citationSuci P, Kang S, Gmu R, Douglas T, Young M, "Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm," Antimicrob Agents, Chemother, 2010 54(6):2489–2496.en_US
dc.identifier.issn0066-4804
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13105
dc.description.abstractThe ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, fluorescently tagged dodecamer. Light-induced activity of the targeted photosensitizer reduced the viability of A. actinomycetemcomitans biofilm, as indicated by membrane permeability to propidium iodide. The functionalized multimeric protein promises to be a useful tool for controlling periodontal biofilm consortia and offers a modular design whereby moieties that target different species can be readily combined with the functionalized protein construct.en_US
dc.titleTargeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilmen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2489en_US
mus.citation.extentlastpage2496en_US
mus.citation.issue6en_US
mus.citation.journaltitleAntimicrobial Agents and Chemotherapyen_US
mus.citation.volume54en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aac.00059-10en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.collegeCollege of Agriculture
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentPlant Sciences & Plant Pathology.
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US


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