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dc.contributor.authorWattanakaroon, Wanida
dc.contributor.authorStewart, Philip S.
dc.identifier.citationWattanakaroon, W. and P.S. Stewart, "Electrical enhancement of streptococcus gordonii biofilm killing by gentamicin," Archives of Oral Biology, 45 (1):167-171 (2000).en_US
dc.description.abstractThis electrical enhancement was demonstrated in an in vitro model. Streptococcus gordonii biofims were grown for 6 days in continuous-flow reactors on one-tenth strength trypticase peptone broth. The biofilms attained a mean areal cell density of 2.4 x 108 c.f.u./cm2 and a thickness of approx. 19 : m. Biofilms exhibited characteristic resistance to killing by an antibiotic. When treated with 2 : g/ml gentamicin for 24 h, they exhibited a 0.84 log reduction in viable cell numbers; a 4.7 log reduction was measured in a planktonic culture. Killing of planktonic bacteria by this treatment was reduced to 1.2 log when an oxygen-scavenging enzyme was added to the medium. When a 2-mA direct current was applied during antibiotic treatment, biofilm killing increased to a 4.3 log reduction. Electrical current alone caused a 1.9 log reduction in biofilm cell counts. It is suggested that gentamicin was less effective against S. Gordonii under anaerobic conditions than it was under aerobic conditions and that this can explain both the reduced susceptibility of the biofilm (due to oxygen depletion) and electrical enhancement of efficacy (due to oxygen generation by electrolysis).en_US
dc.titleElectrical enhancement of streptococcus gordonii biofilm killing by gentamicinen_US
mus.citation.journaltitleArchives of Oral Biologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US

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