Effect of catalase on hydrogen peroxide penetration into pseudomonas aeruginosa biofilms
Date
2000-02Author
Roe, Frank L.
Rayner, Joanna
Elkins, James G.
Lewandowski, Zbigniew
Ochsner, Urs A.
Hassett, Daniel J.
Metadata
Show full item recordAbstract
The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.
Citation
Stewart, P. S., F. Roe, J. Rayner, J. G. Elkins, Z. Lewandowski, U. A. Ochsner, and D. J. Hassett. “Effect of Catalase on Hydrogen Peroxide Penetration into Pseudomonas Aeruginosa Biofilms.” Applied and Environmental Microbiology 66, no. 2 (February 1, 2000): 836–838. doi:10.1128/aem.66.2.836-838.2000.