Show simple item record

dc.contributor.authorCochran, Wendy L.
dc.contributor.authorMcFeters, Gordon A.
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-06-22T13:53:51Z
dc.date.available2017-06-22T13:53:51Z
dc.date.issued2000
dc.identifier.citationCochran W, McFeters G, and Stewart P, "Reduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramine," J Appl Micro, 88 (1):22-30. (2000).en_US
dc.identifier.issn1364-5072
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13129
dc.description.abstractPseudomonas aeruginosa attached to alginate gel beads in sparse, thin biofilms exhibited reduced susceptibility to monochloramine and hydrogen peroxide compared to planktonic cells of the same micro-organism. Disinfection rate coefficients for planktonic bacteria averaged 0.551 mg –1min-1 for monochloramine and 3.1 x 10-4 1 mg –1min-1 for hydrogen peroxide. The corresponding values for 24-h-old biofilm cells were 0.291 mg 1min-1 and 9.2 x 10-5 1 mg –1min-1 for monochloramine and hydrogen peroxide, respectively. Several pieces of evidence support the interpretation that the reduced susceptibility of biofilm was not due simply to inadequate delivery of the antimicrobial agent to the local environment of the attached cells. No correlation between biofilm susceptibility and biofilm initial areal cell density was observed. Rapid delivery of hydrogen peroxide to the attached surface, and subsequently to the interior, of the alginate gel beads was visualized by a direct experimental technique. Theoretical analysis of unsteady diffusion and diffusion-reaction interactions also argued against any significant delay or barrier to antimicrobial or oxygen delivery. It was hypothesized that new genes are expressed when bacteria attach to a surface and begin to form a biofilm, and that some of the resulting gene products reduce the susceptibility of the cell to antimicrobial agents including oxidative biocides such as monochloramine and hydrogen peroxide.en_US
dc.titleReduced susceptibility of thin Pseudomonas aeruginosa biofilms to hydrogen peroxide and monochloramineen_US
dc.typeArticleen_US
mus.citation.extentfirstpage22en_US
mus.citation.extentlastpage30en_US
mus.citation.issue1en_US
mus.citation.journaltitleJournal of Applied Microbiologyen_US
mus.citation.volume88en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.