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dc.contributor.authorArce, Fernando Terán
dc.contributor.authorCarlson, Ross P.
dc.contributor.authorMonds, James
dc.contributor.authorVeeh, Richard Harold
dc.contributor.authorHu, Fen Z.
dc.contributor.authorStewart, Philip Z.
dc.contributor.authorLal, Ratnesh
dc.contributor.authorEhrlich, Garth D.
dc.contributor.authorAvci, Recep
dc.date.accessioned2017-06-26T14:24:38Z
dc.date.available2017-06-26T14:24:38Z
dc.date.issued2009-02
dc.identifier.citationArce FT, Carlson R, Monds J, Veeh R, Hu FZ, Stewart PS, et al. Nanoscale Structural and Mechanical Properties of Nontypeable Haemophilus influenzae Biofilms. Journal of Bacteriology; 2009 Feb 13;191(8):2512–20. DOI: 10.1128/jb.01596-08en_US
dc.identifier.issn0021-9193
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13136
dc.description.abstractNontypeable Haemophilus influenzae (NTHI) bacteria are commensals in the human nasopharynx, as well as pathogens associated with a spectrum of acute and chronic infections. Two important factors that influence NTHI pathogenicity are their ability to adhere to human tissue and their ability to form biofilms. Extracellular polymeric substances (EPS) and bacterial appendages such as pili critically influence cell adhesion and intercellular cohesion during biofilm formation. Structural components in the outer cell membrane, such as lipopolysaccharides, also play a fundamental role in infection of the host organism. In spite of their importance, these pathogenic factors are not yet well characterized at the nanoscale. Here, atomic force microscopy (AFM) was used in aqueous environments to visualize structural details, including probable Hif-type pili, of live NTHI bacteria at the early stages of biofilm formation. Using single-molecule AFM-based spectroscopy, the molecular elasticities of lipooligosaccharides present on NTHI cell surfaces were analyzed and compared between two strains (PittEE and PittGG) with very different pathogenicity profiles. Furthermore, the stiffness of single cells of both strains was measured and subsequently their turgor pressure was estimated.en_US
dc.titleNanoscale Structural and Mechanical Properties of Nontypeable Haemophilus influenzae Biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2512en_US
mus.citation.extentlastpage2520en_US
mus.citation.issue8en_US
mus.citation.journaltitleJournal of Bacteriologyen_US
mus.citation.volume191en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/jb.01596-08en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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