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dc.contributor.authorNocker, Andreas
dc.contributor.authorCamper, Anne K.
dc.date.accessioned2017-06-26T15:35:03Z
dc.date.available2017-06-26T15:35:03Z
dc.date.issued2009-02
dc.identifier.citationNocker Andreas, Camper Anne K. Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiology Letters; 2009 Feb;291(2):137–42. Available from: DOI: 10.1111/j.1574-6968.2008.01429.xen_US
dc.identifier.issn0378-1097
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13142
dc.description.abstractThis article elaborates on possible future directions for microbial viability assessment using nucleic acid-modifying compounds in combination with DNA- (and potentially RNA-) amplification technologies. Bacteria were traditionally considered viable when they could be cultured, whereas today's viability concept is based on the presence of some form of metabolic activity, responsiveness, RNA transcripts that tend to degrade rapidly after cell death, or of an intact membrane. The latter criterion was the focus of recent approaches to limit detection to intact cells using ethidium monoazide or propidium monoazide. Membrane integrity must, however, be considered as a very conservative criterion for microbial viability. The new concept presented here aims at limiting nucleic acid-based detection to cells with an active metabolism, which might be a more appropriate viability criterion. To selectively detect only cells with metabolic and respiratory activity (while excluding inactive dead cells from detection), we suggest the use of ‘activity-labile compounds’. In addition to their potential usefulness for viability assessment, these new compounds could also be beneficial for selectively amplifying nucleic acids of cells that have metabolic activities of interest. This preferential detection of microorganisms with certain metabolic capabilities is referred to as ‘molecular enrichment’ in distinction to ‘growth enrichment’.en_US
dc.titleNovel approaches toward preferential detection of viable cells using nucleic acid amplification techniquesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage137en_US
mus.citation.extentlastpage142en_US
mus.citation.issue2en_US
mus.citation.journaltitleFEMS Microbiology Lettersen_US
mus.citation.volume291en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1111/j.1574-6968.2008.01429.xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage139en_US
mus.contributor.orcidNocker, Andreas|0000-0002-5343-9418en_US


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