Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms
Stewart, Philip S.
Yu, Feipeng Philip
de Beer, Dirk
McFeters, Gordon A.
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Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experienced a 93% reduction. Integrated measures of biofilm respiratory activity, including net oxygen and glucose utilization rates, showed only a 10 to 15% reduction. In this biofilm system, measures of microbial respiratory activity and culturability yielded widely differing estimates of biocide efficacy.
Stewart, P.S., T. Griebe, R. Srinivasan, C.-I. Chen, F.P. Yu, D. DeBeer, and G.A. McFeters, "Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms," Appl. Environ. Microbiol., 60(5):1690-1692.