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dc.contributor.authorDavies, David Gwilym
dc.contributor.authorChakrabarty, Ananda M.
dc.contributor.authorGeesey, Gill G.
dc.identifier.citationDavies DG, Chakrabarty AM, Geesey GG, "Exopolysaccharide production in biofilms: Substratum activation of alginate gene expression by Pseudomonas aeruginosa," Appl Envir Microbiol 1993 59(4):1181–1186.en_US
dc.description.abstractReporter gene technology was employed to detect the activity of an alginate promoter of Pseudomonas aeruginosa when the organism was grown as a biofilm on a Teflon mesh substratum and as planktonic cells in liquid medium. Alginate biosynthetic activity was determined with a mucoid cell line derived from a cystic fibrosis isolate and containing an alginate algC promoter fused to a lacZ reporter gene. Reporter activity was demonstrated with chromogenic and fluorogenic substrates for 13-galactosidase. Expression of algC was shown to be upregulated in biofilm cells compared with planktonic cells in liquid medium. Gene up-expression correlated with alginate biosynthesis as measured by Fourier transform infrared spectroscopy, uronic acid accumulation, and alginate-specific enzyme-linked immunosorbent assay. The algC promoter was shown to have maximum activity in planktonic cultures during the late lag and early log phases of the cell growth cycle. During a time course experiment, biofilm algC activity exceeded planktonic activity except during the period immediately following inoculation into fresh medium. In continuous-culture experiments, conversion of lacZ substrate was demonstrated microscopically in individual cells by epifluorescence microscopy.en_US
dc.titleExopolysaccharide production in biofilms: Substratum activation of alginate gene expression by Pseudomonas aeruginosaen_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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