Bacterial species dominance within a binary culture biofilm
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Date
1991-07
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Abstract
Studies with two species of bacteria, Pseudomonas putida and Hyphomicrobium sp. strain ZV620, were
carried out to evaluate the overall net rate of accumulation of biofilm, the biofilm species composition, and
individual species shear-related removal rates. Bacterial cells of either or both species were deposited onto glass
or biofilm surfaces to initiate multispecies biofilms. Subsequent biofilm development was carried out under
known conditions of nutrient concentration and laminar flow. Establishment of a depositing organism in a
biofllm composed of another species was found to be a function of the relative growth rates of the bacterial
species. In the case of simultaneous species deposition and subsequent binary culture development, the fastergrowing
organisms rapidly became the dominant biofilm species, but the slower-growing organisms remained
established within the biofilm and continued to increase in numbers over time. The results also indicated that
the rate of cell removal by fluid shear for a species was a function of biofilm cell number only if the species
concentration was uniform with depth; in essence, only the upper layers of the biofilm were sheared off.
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Banks, M.K. and J.D. Bryers, "Bacterial species dominance within a binary culture biofilm," Appl. Environ. Microbiol., 57(7):1974-1979 (1991).