Diffusion of Macromolecules in Model Oral Biofilms
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The diffusive penetration of fluorescently tagged macromolecular solutes into model oral biofilms was visualized by time-lapse microscopy. All of the solutes tested, including dextrans, proteases, green fluorescent protein, and immunoglobulin G, accessed the interior of cell clusters 100 to 200 μm in diameter within 3 min or less.
Takenaka S, Pitts B, Trivedi HM, Stewart PS. Diffusion of Macromolecules in Model Oral Biofilms. Applied and Environmental Microbiology; 2009 Jan 23;75(6):1750–3. DOI: 10.1128/aem.02279-08