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dc.contributor.authorSani, Rajesh K.
dc.contributor.authorRastogi, Gurdeep
dc.contributor.authorMoberly, James G.
dc.contributor.authorDohnalkova, Alice
dc.contributor.authorGinn, Timothy R.
dc.contributor.authorSpycher, Nicolas
dc.contributor.authorShende, Rajesh V.
dc.contributor.authorPeyton, Brent M.
dc.date.accessioned2017-07-05T16:37:27Z
dc.date.available2017-07-05T16:37:27Z
dc.date.issued2010-04
dc.identifier.citationSani RK, Rastogi G, Moberly JG, Dohnalkova A, Ginn TR, Spycher N, Shende RV, Peyton, BM, "The toxicity of lead to Desulfovibrio desulfuricans G20 in the presence of goethite and quartz," Journal of Basic Microbiology 2010 50: 160–170en_US
dc.identifier.issn0233-111X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13176
dc.description.abstractAn aqueous mixture of goethite, quartz, and lead chloride (PbCl2) was treated with the sulfate reducing bacterium, Desulfovibrio desulfuricans G20 (D. desulfuricans G20), in a medium specifically designed to assess metal toxicity. In the presence of 26 μM of soluble Pb, together with the goethite and quartz, D. desulfuricans G20 grew after a lag time of 5 days compared to 2 days in Pb-, goethite-, and quartz-free treatments. In the absence of goethite and quartz, however, with 26 μM soluble Pb, no measurable growth was observed. Results showed that D. desulfuricans G20 first removed Pb from solutions then growth began resulting in black precipitates of Pb and iron sulfides. Transmission electron microscopic analyses of thin sections of D. desulfuricans G20 treated with 10 μM PbCl2 in goethite- and quartz-free treatment showed the presence of a dense deposit of lead sulfide precipitates both in the periplasm and cytoplasm. However, thin sections of D. desulfuricans G20 treated with goethite, quartz, and PbCl2 (26 μM soluble Pb) showed the presence of a dense deposit of iron sulfide precipitates both in the periplasm and cytoplasm. Energy-dispersive X-ray spectroscopy, selected area electron diffraction patterns, or X-ray diffraction analyses confirmed the structure of precipitated Pb inside the cell as galena (PbS) in goethite- and quartz-free treatments, and iron sulfides in treatments with goethite, quartz, and PbCl2. Overall results suggest that even at the same soluble Pb concentration (26 μM), in the presence of goethite and quartz, apparent Pb toxicity to D. desulfuricans G20 decreased significantly. Further, accumulation of lead/iron sulfides inside D. desulfuricans G20 cells depended on the presence of goethite and quartz.en_US
dc.titleThe toxicity of lead to Desulfovibrio desulfuricans G20 in the presence of goethite and quartzen_US
dc.typeBook chapteren_US
mus.citation.extentfirstpage160en_US
mus.citation.extentlastpage170en_US
mus.citation.issue2en_US
mus.citation.journaltitleJournal of Basic Microbiologyen_US
mus.citation.volume50en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1002/jobm.200900239en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupEnergy Research Institute.en_US
mus.relation.researchgroupThermal Biology Institute.en_US
mus.data.thumbpage5en_US


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