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dc.contributor.authorPérez-Osorio, Ailyn C.
dc.contributor.authorFranklin, Michael J.
dc.date.accessioned2017-07-06T18:32:39Z
dc.date.available2017-07-06T18:32:39Z
dc.date.issued2008-10
dc.identifier.citationPérez-Osorio AC, Franklin MJ , "qRT-PCR of microbial biofilms," Cold Spring Harb Protoc 2008 3(10)en_US
dc.identifier.issn1940-3402
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13180
dc.description.abstractBacteria growing in biofilms often express a different subset of genes compared to the same strains growing planktonically. Quantitative reverse transcriptase real time PCR (qRT-PCR) can be used effectively to quantify the number of RNA transcripts of specific genes from bacteria growing in biofilms. qRT-PCR has a large dynamic range and may be used to verify gene expression data obtained from microarrays. In addition, qRT-PCR is sensitive, and therefore may be used to quantify gene expression from biofilm samples where only a small amount of biological material is available, as in samples obtained by laser capture microdissection microscopy (LCMM). The most commonly used qRT-PCR methods are the SYBR Green and dual-labeled probe (Taqman) approaches. Both approaches use reverse transcription to convert mRNA to cDNA, followed by PCR amplification of the cDNA. This article describes steps involved in aspects of qRT-PCR including (1) primer design, (2) primer and probe performance testing, (3) qRT-PCR using the Corbett Rotor-Gene system, and (4) data export and analysis.en_US
dc.titleqRT-PCR of microbial biofilmsen_US
dc.typeArticleen_US
mus.citation.issue10en_US
mus.citation.journaltitleCold Spring Harbor Protocolsen_US
mus.citation.volume3en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1101/pdb.prot5066en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage1en_US


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