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dc.contributor.authorPérez-Osorio, Ailyn C.
dc.contributor.authorFranklin, Michael J.
dc.date.accessioned2017-07-06T18:33:26Z
dc.date.available2017-07-06T18:33:26Z
dc.date.issued2008-10
dc.identifier.citationPérez-Osorio AC, Franklin MJ, "Isolation of RNA and DNA from biofilm samples obtained by laser capture microdissection microscopy," Cold Spring Harb Protoc 2008 3(10)en_US
dc.identifier.issn1940-3402
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13181
dc.description.abstractThe metabolic activities of bacteria growing in biofilms result in spatial gradients of oxygen, nutrients, and waste products. Because bacteria respond to local environmental conditions through changes in gene expression, mRNA levels of individual genes may vary spatially among bacteria within the biofilm. This article describes an approach to isolate RNA for quantification from cells at localized sites within biofilms. Biofilm thin sections are generated by embedding biofilms in cryoembedding resin, freezing the embedded biofilms on dry ice, and cutting with a cryomicrotome. The sections are placed on membrane-coated microscope slides and maintained on dry ice. Laser capture microdissection microscopy (LCMM) is used to dissect small subsets of cells at different regions within the biofilms, and RNA is extracted from the samples using either hot phenol or TRI reagent. A TRI reagent-based DNA extraction method is also presented.en_US
dc.titleIsolation of RNA and DNA from biofilm samples obtained by laser capture microdissection microscopyen_US
dc.typeArticleen_US
mus.citation.issue10en_US
mus.citation.journaltitleCold Spring Harbor Protocolsen_US
mus.citation.volume3en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1101/pdb.prot5065en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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