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dc.contributor.authorNaumenko, E. A.
dc.contributor.authorNaumov, A. V.
dc.contributor.authorSuvorova, E. S.
dc.contributor.authorGerlach, Robin
dc.contributor.authorZiganshin, Ayrat M.
dc.contributor.authorLozhkin, A. P.
dc.contributor.authorSilkin, N. I.
dc.contributor.authorNaumova, R. P.
dc.date.accessioned2017-07-07T16:02:43Z
dc.date.available2017-07-07T16:02:43Z
dc.date.issued2008-04
dc.identifier.citationNaumenko EA, Naumova AV, Suvorova ES, Gerlach R, Ziganshin AM, Lozhkin AP, Silkin NI, Naumova RP, "Participation of oxygen in the bacterial transformation of 2,4,6-trinitrotoluene," Biochemistry (Moscow) 73(4):463-469 (2008)en_US
dc.identifier.issn0006-2979
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13192
dc.description.abstractThe exposure of Bacillus cereus ZS18 cell suspensions to 2,4,6-trinitrotoluene (TNT) in the absence of other oxidizable substrates increases oxygen uptake, exceeding the basal level of respiration of the bacterium 1.5- and 2-fold with 50 and 100 mg/liter of TNT, respectively. The interaction of both living and to less extent dead bacterial cells with TNT results in the accumulation of superoxide anion (O2-) in the extracellular medium, which was revealed by the EPR spectroscopy. The accumulation of O2- decreased by 50-70% in the presence of Cu,Zn-superoxide dismutase of animal origin. In the presence of living bacterial cells, the level of TNT decreased progressively, yielding hydroxylaminodinitrotoluenes together with O2-. In the presence of heat-killed cells, a moderate decrease in TNT was observed, and the appearance of O2- was not accompanied by the production of any detectable TNT metabolites. Chelating agents inhibited the transformation of TNT and decreased the formation of O2-. The demonstrated generation of O2- during the interaction of TNT with K4[Fe(CN)6] together with the observed effects of chelating agents suggest the participation of iron in the one-electron reduction of TNT and the functioning of an extracellular redox cycle with the involvement of molecular oxygen.en_US
dc.titleParticipation of oxygen in the bacterial transformation of 2,4,6-trinitrotolueneen_US
dc.typeArticleen_US
mus.citation.extentfirstpage463en_US
mus.citation.extentlastpage469en_US
mus.citation.issue4en_US
mus.citation.journaltitleBiochemistry (Moscow)en_US
mus.citation.volume73en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1134/s0006297908040123en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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